Plant Tissue Culture Definition: The term plant tissue culture is described in vitro and aseptic cultivation of any plant part on nutrient medium.
Date added: March 30, 2013 - Views: 265
Plant Tissue Culture in vitro propagation Rapid (compared to outdoors) Asexual Used for 1. Micropropagation 2. Regeneration Vegetative (asexual) Propagation Occurs in Nature Layering - a drooping lower branch contacts the soil (pressed down by snow or vegetation); roots form at point of soil ...
Date added: September 15, 2011 - Views: 226
Our Learning Objectives in relation to Tissue Culture. Reasons for propagating plants by tissue culture are stated. The culture process is described
Date added: January 30, 2012 - Views: 456
Major development’s in cell culture technology Why is cell culture used for? Contd…. Tissue culture Primary culture Continous cell lines Types of cells Culture media Why sub culturing.? Culturing ...
Date added: September 24, 2011 - Views: 468
Plant Tissue Culture T.C. Refers to technique of growing plant cells, tissues, organs, seeds or other plant parts in a sterile environment on a nutrient medium History In 1902 Haberlandt proposed that single plant cells could be cultured Haberlandt did not culture them himself 1930’s White ...
Date added: February 28, 2012 - Views: 358
Title: Cell & Tissue Culture Author: Pooja- Keshav Last modified by: Doddy Created Date: 9/17/2000 8:18:18 AM Document presentation format: On-screen Show
Date added: November 27, 2012 - Views: 253
Cell And Tissue Culture Mammalian Cells Advanced Higher Biology Why is it useful? Gene manipulation Culturing mammalian cells for cancer studies Producing new plants through tissue culture What do you need to do it?
Date added: October 26, 2011 - Views: 252
Tissue Culture Methodology Historical Perspective Early 20th Century Technique Antibiotics and Laminar Flow Hoods Allow for Tissue Culturing to Grow Exponentially Unfortunately NOT commonly taught in a classroom setting Outline Primary Cells vs Cell lines Acquisition of Specimens Processing ...
Date added: August 28, 2011 - Views: 67
Plant Tissue Culture Project Original Power Point Presentation by Paige and Elisa Modified by GA Agricultural Education Curriculum Office July 2002
Date added: February 14, 2012 - Views: 36
Plant Tissue Culture What is it? Tissue culture is the term used for “the process of growing cells artificially in the laboratory” Tissue culture produces clones, in which all product cells have the same genotype (unless affected by mutation during culture) Cell theory, suggesting ...
Date added: September 11, 2012 - Views: 44
Plant Tissue Cultures Conditions required for plant tissue cultures An original stock of cells. The use of aseptic techniques when preparing media and cultures.
Date added: May 5, 2013 - Views: 67
Plantain, banana and pineapple tissue culture Biochemical Products Ghana Ltd Ningo Prampram Road, New Ningo, Dangme West District, Greater Accra,
Date added: November 27, 2013 - Views: 7
Plant Tissue Culture What Is plant tissue culture? Definition Basis for Plant Tissue Culture Two Hormones Affect Plant Differentiation: Auxin: Stimulates Root Development Cytokinin: Stimulates Shoot Development Generally, the ratio of these two hormones can determine plant development: Auxin ...
Date added: August 27, 2013 - Views: 124
Grow mammalian vs bacterial cells. For both need: For bacteria: Tryptone, yeast extract, salt +/- antibiotic, agar. For MES cells: DMEM, calf serum, glutamine, penn/strep
Date added: September 9, 2013 - Views: 3
Plant Tissue Culture-generation of whole plants from a single cell or tissue-Biotech II * * * Picture in back of your protocol packet shows detail re: how the amplification works.
Date added: November 9, 2013 - Views: 23
Growing Cells in Culture Part 1: Terminology Some volumes that do not need to be exact—but follow our recommendations until you are comfortable Rinsing volume of PBS Volume of trypsin EDTA Volume of media to resuspend cells Record how much Volume of cells removed for counting Exact # of cells ...
Date added: October 19, 2011 - Views: 111
Introduction to Tissue culture Experimental Methods 2006 * Temperature The optimum temperature depends on the body temperature of animals from which the cells were obtained anatomical variation of temperature (skin temperature may be lower than the rest of the body) Experimental Methods 2006 ...
Date added: May 3, 2013 - Views: 42
What is Plant Tissue Culture? Anther and microspore culture Production of haploid plants Production of homozygous diploid lines through chromosome doubling, ...
Date added: September 18, 2011 - Views: 74
Oron Catts & Ionat Surr: Tissue Culture & Art Project Tissue Culture: (Wikipedia) the growth of tissues and/or cells separate from the organism. This is typically facilitated via use of a liquid, semi-solid, or solid growth medium, such as broth or agar.
Date added: October 23, 2011 - Views: 56
Plant Propagation Jeff Schalau Assoc. Agent, Agriculture & Natural Resources University of Arizona, ... media/manipulate to root and induce juvenile state Grow in sterile culture and propagate Transfer to nursery containers Tissue Culture ...
Date added: May 3, 2013 - Views: 23
Basic parts of bioreactor Bioreactors for cultivation of plant cells Slide 20 Slide 21 Slide 22 Plant Tissue Culture- Different Approaches for Production of Secondary Metabolites Induction of Hairy Roots by Agrobacterium rhizogenes Advantages of Hairy Root Culture Over Plant Cell ...
Date added: May 13, 2012 - Views: 167
Plant Tissue Culture & Applications Basis for Plant Tissue Culture Two Hormones Affect Plant Differentiation: Auxin: Stimulates Root Development Cytokinin: Stimulates Shoot Development Generally, the ratio of these two hormones can determine plant development: Auxin ↓Cytokinin = Root ...
Date added: June 3, 2013 - Views: 33
The rate of variation expected in tissue culture is approximately 10-15% which is very high in comparison to in vivo systems. The plants regenerated from such callus can be tested for heritability, expressivity and stability and subjected to different levels of field trials.
Date added: May 2, 2013 - Views: 187
Plant Biology and Production Unit 5 Lesson 3 Propagating Plants by Tissue Culture Vocabulary Auxins Callus Cytokinins Explants Plantlet Sterile agar medium Sterile technique Tissue culture Student Objectives 1.
Date added: June 17, 2013 - Views: 13
Genetic variations in plants that have been produced by plant tissue culture and can be detected as genetic or phenotypic traits. Basic Features of Somaclonal Variations Variations for Karyotype, isozyme characteristics and morphology in somaclones may also observed. ...
Date added: November 21, 2011 - Views: 203
ANTHER CULTURE Anther culture id a technique by which the developing anthers at a precise and critical stage are excised aseptically from unopened flower bud and are cultured on a nutrient medium where the microspores within the cultured anther develop into callus tissue or embryoids that give ...
Date added: September 20, 2012 - Views: 219
Conducting tissue culture in the regular laboratory area should be avoided; ... trypsin) that has been used in conjunction with the culture. (5) If the contamination is new and widespread (i.e., in at least two different cultures), discard all media, stock solutions, trypsin, etc. (6) ...
Date added: August 18, 2013 - Views: 4
... Is Tissue Culture Treated With High Energy Ionizing Radiation Electric Ion Discharge Adhesion Is Mediated By Surface Receptors And Matrix Matrix Is Secreted By Cells, ...
Date added: November 11, 2011 - Views: 21
Plant Propagation by Tissue Culture GJ DeKlerk, EF George, MA Hall (eds) 3rd Edition, 2008 Springer http://www.springerlink.com/content/n5tm30/?p=2d1b86aedb5a437f987c7bd63962bbc8&pi=0 mericloning A propagation method using shoot tips in culture to proliferate multiple buds, ...
Date added: September 8, 2012 - Views: 25
Producing secondary metabolites in tissue culture may have a negative impact on the economics of the Third World countries Many of these Third World countries may lose market share to superior, more efficient production of secondary metabolites in industrial countries.
Date added: January 27, 2012 - Views: 72
Plant and Mammalian Tissue Culture Mammalian Cell Culture * * * * * * Hayflick’s Phenomenon and Aging There appears to be a correlation between the maximal number of passages and aging.
Date added: May 23, 2013 - Views: 8
Monoclonal Antibody Production Cathy Langford, Judy Knadler, ... method) or by in vitro tissue culture. The vitro tissue culture is the method used when the cells are places in culture outside the mouse's body in a flask.
Date added: October 26, 2011 - Views: 139
Title: An insight into the tissue culture technique and its implication Author: Samina Haq Last modified by: Dr.Gamal Created Date: 5/12/2008 6:45:03 AM
Date added: May 19, 2012 - Views: 75
Brief History of Tissue Culture. 1885. Wilhelm Roux. Embryonic chick cells maintained alive in a . saline solution. 1907. Ross Harrison. Frog Embryo nerve fibre
Date added: April 21, 2014 - Views: 7
Plant and Mammalian Tissue Culture Plant Cell Culture Plant Tissue Culture Method of asexually growing new plant material A way to replicate plants “in vitro” Differs from conventional propagation using cuttings or grafting Can be started from mature plants, tissues or plant organs Highly ...
Date added: August 2, 2013 - Views: 22
Somaclonal variation It is the term used to describe the variation seen in plants that have been produced by plant tissue culture. Chromosomal rearrangements are an important source of this variation.
Date added: May 22, 2013 - Views: 52
Introduction. What is Plant Tissue Culture? Plant tissue culture is the aseptic (free from microorganism) culture of any plant part in vitro on an artificially prepared nutrient media and under controlled conditions.
Date added: May 2, 2013 - Views: 84
Plant Breeding and Applied Genetics Ute Achenbach ... 11 Classical Plant Breeding (Breeding schemes, e.g. Peach) Jun 17 Applied Plant Breeding July 1 Molecular Genetics (Tissue culture, e.g. grapes) (Dr. Sadanand Dhekney) July 8 Molecular Genetics (PCR, Different marker systems) ...
Date added: May 2, 2013 - Views: 117
Title: Modern Methods for Influenza Detection and Sub-typing Last modified by: wangsnk Created Date: 12/2/2004 8:27:39 PM Document presentation format
Date added: January 25, 2013 - Views: 19
Laboratory Diagnosis of Viral Infection Detection – Isolation - Serology ... Virus isolation in tissue culture cell line Viral identification: This is achieved by: (a) The effect on cell culture: i.e. cytopathic effect, (b) ...
Date added: May 31, 2012 - Views: 95
Introduction to Tissue culture Sompol Tapechum M.D., Ph.D. Department of Physiology Faculty of Medicine Siriraj Hospital Objectives After the session, students should be able to explain the meaning of tissue culture and various types of tissue culture the application of tissue culture the ...
Date added: May 4, 2013 - Views: 23
Propagating Plants by Tissue Culture Vocabulary Auxins Callus Cytokinins Explants Plantlet Sterile agar medium Sterile technique Tissue culture Student Objectives 1.
Date added: July 16, 2014 - Views: 1
Cell Culture Dr. Tarek Elbashiti Assoc. Prof. of Biotechnology The term cell culture refers to the cultivation of dispersed (isolated) cells taken from an original tissue, a primary culture, or a cell line.
Date added: September 26, 2013 - Views: 13
TISSUE CULTURE MAJOR COMPONENTS OF TISSUE CULTURE MEDIA STERILIZATION METHODS ... 1. Surface Sterilization of the Explant 2. Preparation of Nutrient Medium 3. Inoculation 4. Callus Growth 5. Subculturing 6. Organogenesis 7. Direct Regeneration 8.
Date added: February 23, 2014 - Views: 16
... 1998 East and central Africa Burundi Democratic Republic of Congo production of rhizobial-based bioferttilizers Kenya tissue culture of medicinal plants production of disease free plants in vitro selection for salt resistance in finger millet in vitro long-term storage of potato and ...
Date added: October 6, 2011 - Views: 202
Virology Lab 6 Definitive Properties of Viruses An infectious, ... and breaking of chromosomes Rounding up and detachment of cultured cells Inclusion bodies Quantification of CPE Tissue Culture Infective Dose 50 (TCID50): ...
Date added: October 15, 2011 - Views: 66
The Strecker Memorandum A Review & Commentary Use your arrow keys to navigate ... Further, it is prolific in tissue culture. The same characteristics as HTLV-1 HTLV-2 & Bovine Syncytial Also in cattle is a virus known as bovine Syncytial virus (or BSV).
Date added: April 9, 2012 - Views: 39
Plant propagation... Sexual methods - seed propagation Asexual methods rooting cuttings layering; air layering grafting specialized structures tissue culture; micropropagation Asexual propagation...
Date added: October 12, 2011 - Views: 180