Plant Tissue Culture Definition: The term plant tissue culture is described in vitro and aseptic cultivation of any plant part on nutrient medium.
Date added: March 30, 2013 - Views: 269
Title: Tissue Culture And Micro Propagation Author: Lawrence F. Iles Last modified by: b715324 Created Date: 9/17/2004 2:36:40 AM Document presentation format
Date added: September 15, 2011 - Views: 230
Major development’s in cell culture technology Why is cell culture used for? Contd…. Tissue culture Primary culture Continous cell lines Types of cells Culture media Why sub culturing.?
Date added: September 24, 2011 - Views: 477
Cell And Tissue Culture Mammalian Cells Advanced Higher Biology Why is it useful? Gene manipulation Culturing mammalian cells for cancer studies Producing new plants through tissue culture What do you need to do it?
Date added: October 26, 2011 - Views: 256
Our Learning Objectives in relation to Tissue Culture. Reasons for propagating plants by tissue culture are stated. The culture process is described
Date added: January 30, 2012 - Views: 460
Plant Tissue Culture Project Original Power Point Presentation by Paige and Elisa Modified by GA Agricultural Education Curriculum Office July 2002 What is Plant Tissue Culture? Plant tissue culture is a form of asexual propagation of plants under laboratory conditions.
Date added: February 14, 2012 - Views: 37
Tissue Culture Methodology Historical Perspective Early 20th Century Technique Antibiotics and Laminar Flow Hoods Allow for Tissue Culturing to Grow Exponentially Unfortunately NOT commonly taught in a classroom setting Outline Primary Cells vs Cell lines Acquisition of Specimens Processing ...
Date added: August 28, 2011 - Views: 70
Title: Cell & Tissue Culture Author: Pooja- Keshav Last modified by: Doddy Created Date: 9/17/2000 8:18:18 AM Document presentation format: On-screen Show
Date added: November 27, 2012 - Views: 261
Grow mammalian vs bacterial cells. For both need: For bacteria: Tryptone, yeast extract, salt +/- antibiotic, agar. For MES cells: DMEM, calf serum, glutamine, penn/strep
Date added: September 9, 2013 - Views: 3
Tissue culture provided another level of genetic variation: somaclonal variation. Ability to cut and ligate DNA: gene mapping and cloning techniques. Ability to introduce foreign DNA that ends up in the nucleus and ligates with the native DNA.
Date added: May 2, 2013 - Views: 76
Plantain, banana and pineapple tissue culture Biochemical Products Ghana Ltd Ningo Prampram Road, New Ningo, Dangme West District, Greater Accra,
Date added: November 27, 2013 - Views: 9
Plant Tissue Culture Original by Linda Rist Modified by Georgia Agricultural Education Curriculum Office July, 2002 T.C. Refers to technique of growing plant cells, tissues, organs, seeds or other plant parts in a sterile environment on a nutrient medium History In 1902 Haberlandt proposed that ...
Date added: May 2, 2013 - Views: 14
Plant Tissue Cultures Conditions required for plant tissue cultures An original stock of cells. The use of aseptic techniques when preparing media and cultures.
Date added: May 5, 2013 - Views: 72
Plant Tissue Culture What is it? Tissue culture is the term used for “the process of growing cells artificially in the laboratory” Tissue culture produces clones, in which all product cells have the same genotype (unless affected by mutation during culture) Cell theory, suggesting ...
Date added: September 11, 2012 - Views: 47
Growing Cells in Culture Part 1: Terminology Some volumes that do not need to be exact—but follow our recommendations until you are comfortable Rinsing volume of PBS Volume of trypsin EDTA Volume of media to resuspend cells Record how much Volume of cells removed for counting Exact # of cells ...
Date added: October 19, 2011 - Views: 115
Plant Tissue Culture-generation of whole plants from a single cell or tissue-Biotech II * * * Picture in back of your protocol packet shows detail re: how the amplification works.
Date added: November 9, 2013 - Views: 26
... Is Tissue Culture Treated With High Energy Ionizing Radiation Electric Ion Discharge Adhesion Is Mediated By Surface Receptors And Matrix Matrix Is Secreted By Cells, ...
Date added: November 11, 2011 - Views: 26
What is Plant Tissue Culture? Anther and microspore culture Production of haploid plants Production of homozygous diploid lines through chromosome doubling, ...
Date added: September 18, 2011 - Views: 76
Oron Catts & Ionat Surr: Tissue Culture & Art Project Tissue Culture: (Wikipedia) the growth of tissues and/or cells separate from the organism. This is typically facilitated via use of a liquid, semi-solid, or solid growth medium, such as broth or agar.
Date added: October 23, 2011 - Views: 60
Plant Tissue Culture What Is plant tissue culture? Definition Basis for Plant Tissue Culture Two Hormones Affect Plant Differentiation: Auxin: Stimulates Root Development Cytokinin: Stimulates Shoot Development Generally, the ratio of these two hormones can determine plant development: Auxin ...
Date added: August 27, 2013 - Views: 133
Plant Propagation Jeff Schalau Assoc. Agent, Agriculture & Natural Resources University of Arizona, ... media/manipulate to root and induce juvenile state Grow in sterile culture and propagate Transfer to nursery containers Tissue Culture ...
Date added: May 3, 2013 - Views: 25
Plant Biology and Production Unit 5 Lesson 3 Propagating Plants by Tissue Culture Vocabulary Auxins Callus Cytokinins Explants Plantlet Sterile agar medium Sterile technique Tissue culture Student Objectives 1.
Date added: June 17, 2013 - Views: 17
Plant Tissue Culture & Applications Basis for Plant Tissue Culture Two Hormones Affect Plant Differentiation: Auxin: Stimulates Root Development Cytokinin: Stimulates Shoot Development Generally, the ratio of these two hormones can determine plant development: Auxin ↓Cytokinin = Root ...
Date added: June 3, 2013 - Views: 37
Basic parts of bioreactor Bioreactors for cultivation of plant cells Slide 20 Slide 21 Slide 22 Plant Tissue Culture- Different Approaches for Production of Secondary Metabolites Induction of Hairy Roots by Agrobacterium rhizogenes Advantages of Hairy Root Culture Over Plant Cell ...
Date added: May 13, 2012 - Views: 171
Genetic variations in plants that have been produced by plant tissue culture and can be detected as genetic or phenotypic traits. Basic Features of Somaclonal Variations Variations for Karyotype, isozyme characteristics and morphology in somaclones may also observed. Calliclone (clones of ...
Date added: November 21, 2011 - Views: 208
Plant Tissue Culture Plant Tissue Culture? Definition Basis for Plant Tissue Culture Two Hormones Affect Plant Differentiation: Auxin: Stimulates Root Development Cytokinin: Stimulates Shoot Development Generally, the ratio of these two hormones can determine plant development: Auxin ...
Date added: August 28, 2013 - Views: 11
Introduction to Tissue culture Experimental Methods 2006 * Temperature The optimum temperature depends on the body temperature of animals from which the cells were obtained anatomical variation of temperature (skin temperature may be lower than the rest of the body) Experimental Methods 2006 ...
Date added: May 3, 2013 - Views: 46
Introduction to Tissue culture Sompol Tapechum M.D., Ph.D. Department of Physiology Faculty of Medicine Siriraj Hospital Objectives After the session, students should be able to explain the meaning of tissue culture and various types of tissue culture the application of tissue culture the ...
Date added: May 4, 2013 - Views: 23
Plant Propagation by Tissue Culture GJ DeKlerk, EF George, MA Hall (eds) 3rd Edition, 2008 Springer http://www.springerlink.com/content/n5tm30/?p=2d1b86aedb5a437f987c7bd63962bbc8&pi=0 mericloning A propagation method using shoot tips in culture to proliferate multiple buds, ...
Date added: September 8, 2012 - Views: 28
Conducting tissue culture in the regular laboratory area should be avoided; ... trypsin) that has been used in conjunction with the culture. (5) If the contamination is new and widespread (i.e., in at least two different cultures), discard all media, stock solutions, trypsin, etc. (6) ...
Date added: August 18, 2013 - Views: 6
Producing secondary metabolites in tissue culture may have a negative impact on the economics of the Third World countries PowerPoint Presentation PowerPoint Presentation ...
Date added: January 27, 2012 - Views: 74
Monoclonal Antibody Production or mAb is produced by cell lines or clones obtained from the immunized animals with the substance to be studied. ... The vitro tissue culture is the method used when the cells are places in culture outside the mouse's body in a flask.
Date added: October 26, 2011 - Views: 143
Brief History of Tissue Culture. 1885. Wilhelm Roux. Embryonic chick cells maintained alive in a . saline solution. 1907. Ross Harrison. Frog Embryo nerve fibre
Date added: April 21, 2014 - Views: 8
The rate of variation expected in tissue culture is approximately 10-15% which is very high in comparison to in vivo systems. The plants regenerated from such callus can be tested for heritability, expressivity and stability and subjected to different levels of field trials.
Date added: May 2, 2013 - Views: 191
Propagating Plants by Tissue Culture Vocabulary Auxins Callus Cytokinins Explants Plantlet Sterile agar medium Sterile technique Tissue culture Student Objectives 1.
Date added: July 16, 2014 - Views: 1
Introduction. What is Plant Tissue Culture? Plant tissue culture is the aseptic (free from microorganism) culture of any plant part in vitro on an artificially prepared nutrient media and under controlled conditions.
Date added: May 2, 2013 - Views: 84
... histology sections Record values and observations in lab notebook Observe cell monolayer on inverted scope Live tissue cells to be cultured Culture vessel with appropriate growth media Cell adhere to vessel and grow to form a monolayer Lift cells into solution with enzyme Seed cells ...
Date added: October 15, 2011 - Views: 66
Plant propagation... Sexual methods - seed propagation Asexual methods rooting cuttings layering; air layering grafting specialized structures tissue culture; micropropagation Asexual propagation...
Date added: October 12, 2011 - Views: 185
... 1998 East and central Africa Burundi Democratic Republic of Congo production of rhizobial-based bioferttilizers Kenya tissue culture of medicinal plants production of disease free plants in vitro selection for salt resistance in finger millet in vitro long-term storage of potato and ...
Date added: October 6, 2011 - Views: 207
An Ecological Perspective (BIOL 346) Talk Seven: GMOs and the Environment And then?..... What is the last step?..... Tissue culture The basics!
Date added: December 20, 2013 - Views: 14
Plant and Mammalian Tissue Culture Plant Culture Laboratory - Start Flytrap or Rose Culture Lab Goals Become familiar with Plant Tissue Culture Work aseptically with plant culture Maintain cultures for an extended period of time Choose Either Rose or Venus’ Flytrap to use for Micropropagation ...
Date added: June 12, 2013 - Views: 5
Mammalian Cell Culture What is cell culture, exactly? Cells, previously growing in a human or animal modified to grow in plastic or glass In the body = in vivo On plastic or glass = in vitro Kept in an incubator to stay at body temperature We use special media with nutrients so the cells can ...
Date added: November 20, 2011 - Views: 203
TISSUE CULTURE MAJOR COMPONENTS OF TISSUE CULTURE MEDIA STERILIZATION METHODS ... 1. Surface Sterilization of the Explant 2. Preparation of Nutrient Medium 3. Inoculation 4. Callus Growth 5. Subculturing 6. Organogenesis 7. Direct Regeneration 8.
Date added: February 23, 2014 - Views: 16
... Epistasis) Jun 11 Classical Plant Breeding (Breeding schemes, e.g. Peach) Jun 17 Applied Plant Breeding July 1 Molecular Genetics (Tissue culture, e.g ... tools Mutagenesis Tissue culture Haploidy In situ hybridization DNA ... Learning outcomes PowerPoint Presentation ...
Date added: May 2, 2013 - Views: 117
Title: Tissue Culture Author: ITS Last modified by: srm Created Date: 6/4/2003 3:05:55 AM Document presentation format: On-screen Show Company: Massey University
Date added: January 20, 2013 - Views: 170
Principles of Cell Culture Hessah Alshammari * * * * * * * * Cell Culture in vitro - A brief history 1885: Roux maintained embryonic chick cells alive in saline solution for short lengths of time 1912: Alexis Carrel cultured connective tissue and showed heart muscle tissue contractility over 2-3 ...
Date added: May 2, 2013 - Views: 14