SOUTHERN BLOTTING The technique was developed by E.M. Southern in 1975. ... Would you watch the animation? A. Yes B. No SUMMARY OF PROCEDURE 1. Extract and purify DNA from cells 2. DNA is restricted with enzymes 3. Sort by electrophoresis 4. Denature DNA 5. Transfer to nitrocellulose paper 6.
Date added: June 25, 2012 - Views: 158
Introduction to Southern Hybridization Michael Melzer Plant & Environmental Protection Sciences University of Hawaii at Manoa Outline History/Background Info Goals of Southern hybridization Example Other applications History/Background ‘Southern’ hybridization named after Sir Edwin Southern ...
Date added: November 6, 2011 - Views: 216
Southern Blotting INVENTED BY BRITISH BIOCHEMIST EDWIN SOUTHERN * Purpose Used to determine whether a particular nucleotide sequence is present in different sources of DNA, and also to discover the size of the restriction fragment that contains the specific sequence Example Sickle Cell Can ...
Date added: November 28, 2011 - Views: 234
Southern Blotting A method to visualize specific segments of DNA– usually a particular gene. Uses radioactive probes that bind to the specific DNA segments Ex. ... DNA is completely unzipped, no Okazaki fragments PCR animation animation Genetics Techniques: ...
Date added: April 15, 2012 - Views: 298
Size determined by gel electrophoresis and Southern blotting to produce pattern of ... Activities Review tutorial on Western Blot http://tinyurl.com/awaut3 Review VNTR animation http://tinyurl.com/dawjau Review DNA fingerprinting http://tinyurl.com/co2yml Review DNA forensics Problem set 1 ...
Date added: September 13, 2011 - Views: 165
Steps in Southern Blotting Southern Blotting Animation Advantages and Disadvantages of Southern Blotting Advantages The exact location of a disorder does not need to be known Disadvantages They are rather time consuming Needs high quality/quantity DNA Radioactive Some tasks previously ...
Date added: March 29, 2013 - Views: 5
Using living microorganisms or cell components to make products Often via genetic engineering and using ... Autoradiography pinpoints sequence Southern Blot animation The scientific applications Understanding of DNA ... is gel electrophoresis? What is Southern blotting?
Date added: May 22, 2013 - Views: 19
Figure 20.9 Gel electrophoresis Figure 20.9 Gel electrophoresis Figure 20.11 Southern blotting of DNA fragments Figure ... allele Probe base-pairs with fragments Nitrocellulose blot 4 5 Radioactively labeled probe for -globin gene Southern blotting animation Northern blotting ...
Date added: September 4, 2011 - Views: 101
DNA technology the study of sequence, expression, and function of a gene DNA cloning allows researchers to Compare genes and alleles between individuals
Date added: May 4, 2013 - Views: 15
Scene: Animation of labeled probe fragment pairing with target ... Animation of DNA/RNA probe preparations Animation of Complementary Base-Pairing Live shot of Researcher--Segue to Blotting Southern Blot Transfer Preparation Animation of Transfer Process Crosslink of DNA to Nitrocellulose ...
Date added: November 7, 2011 - Views: 30
... DNA Sequencing organisms' genomes DNA fingerprinting for identification Southern Blotting Southern Blotting Southern Blotting Forensic Microbiology PCR Primer for a specific organism will cause application if ... Recombinant DNA Technology Table 9.1 Figure 9.2 Figure 9.3 ANIMATION PCR: ...
Date added: October 26, 2011 - Views: 76
Web animation of a cDNA microarray experiment Yeast genome on a chip Brief outline of steps for ... (e.g. by X-ray for radioactive probes) Sample Autoradiogragh (Gel) Types of Blots Southern ... 3’ 3’ – CTTAAG – 5’ (cut) The Southern blotting technique * Title ...
Date added: October 15, 2011 - Views: 94
... the role of a gene in an organism Several techniques are used to analyze the DNA of genes Gel Electrophoresis and Southern Blotting One indirect method of rapidly analyzing and comparing genomes is gel electrophoresis This technique uses a gel as a ... Base pairing occurs. 2 Animation: ...
Date added: May 13, 2013 - Views: 11
... shorter and less susceptible to degradation, first loci to be used in forensic analysis Short Tandem Repeats repeats of 2 to 7 bases ... This would take about 3 hours to perform in lab. PCR Animation DNA Fingerprinting Used to identify individuals by their ... (Southern blotting) ...
Date added: February 13, 2013 - Views: 34
Chapter 20 Biotechnology ... and sickle-cell alleles of the β-globin gene * * Figure 20.11 Southern blotting of DNA fragments * Figure 20.11 Southern blotting of DNA fragments * Figure 20 ... producing fragments with “sticky ends” that bond with complementary sticky ends of other fragments ...
Date added: May 3, 2014 - Views: 5
* * Figure 20.11 Research Method: Southern Blotting of DNA Fragments * * Figure 20.12 Research Method: ... The fragments are mixed, and DNA ligase is added to bond the fragment sticky ends © 2011 Pearson Education, Inc. Animation: ...
Date added: March 16, 2013 - Views: 12
Examples of Applications: Southern blotting, DNA chips, and FISH Figs. 10.17 and 10.18 PLAY Animation: Dichotomous Keys (The Microbiology Place) Method used to establish cladogram for microorganisms? ...
Date added: June 12, 2012 - Views: 65
PBIO 427/527: Molecular Genetics Lecture 3 - Review Southern, Northern & Western blotting DNA sequencing Polymerase chain reaction (PCR) DNA microarrays
Date added: May 4, 2013 - Views: 6
Examples of Applications: Southern blotting, DNA chips, and FISH Nucleic Acid Hybridization: DNA Chip Fig 10.17 Fluorescent In Situ Hybridization (FISH) Fig 10 ... Practice ANIMATION Dichotomous Keys: ...
Date added: December 5, 2011 - Views: 74
Several techniques are used to analyze the DNA of genes: Gel Electrophoresis, restriction fragment analysis, Southern blotting, DNA sequencing. ... Figure 20.18 Reproductive cloning of a mammal by nuclear transplantation For the Discovery Video Cloning, go to Animation and Video Files.
Date added: February 9, 2012 - Views: 35
Hybridization DNA hybridization Southern blotting (pg75) Southern Blot ... Primer extension Fig. 3.8 The Polymerase Chain Reaction Go to Animation courtesy of the following websites: References http://faculty.plattsburgh.edu/donald.slish/PCRmov.html ...
Date added: July 1, 2012 - Views: 19
DNA Technology AP Biology ... Biology Animation Library/DNA Restriction Restriction enzymes are used to make recombinant DNA Restriction enzymes cut both strands, often in a staggered way creating single-stranded ends, ... Southern Blotting: ...
Date added: April 23, 2014 - Views: 4
... t tell you it’s classified as gammaproteobacteria References What is in Bergey’s Manual? 10-13 A Clinical Microbiology Lab Report Form Identification ... Cladogram Building a Cladogram What is tested in Western blotting and Southern ... ANIMATION Dichotomous Keys ...
Date added: May 3, 2013 - Views: 37
... Scientific Applications Understanding DNA Sequencing organisms' genomes DNA fingerprinting for identification Southern Blotting Southern Blotting Southern Blotting Forensic Microbiology PCR Primer for a specific ... Recombinant DNA Technology Table 9.1 Figure 9.2 Figure 9.3 ANIMATION ...
Date added: November 27, 2013 - Views: 4
Restriction Fragment Length ... and gel electrophoresis DNA transfer by Southern blotting DNA hybridisation Interpreting RFLP bands Advantages and disadvantages of RFLPs ... for choosing a RFLP marker Slide 14 Summary Animation RFLP Analysis Restriction enzymes ...
Date added: May 4, 2013 - Views: 16
Polymerase Chain Reaction Animation PCR http://www.dnalc.org/ddnalc/resources/pcr.html Gel electrophoresis a method that ... http://220.127.116.11/pub/flash/4/4.html `http://www.dnalc.org/ddnalc/resources/electrophoresis.html Southern blotting Southern blotting was named after Edward M ...
Date added: March 22, 2012 - Views: 33
... 9-16 Applications of rDNA 9-17 Diagram the Southern blotting ... filter DNA transferred to filter Gel Figure 9.16 Southern blotting. Forensic Microbiology PCR Primer for a specific organism will cause ... Sequence DNA Diagnose genetic disease Detect pathogens ANIMATION ...
Date added: May 2, 2013 - Views: 29
Southern blotting c. Northern blotting Restriction Mapping Animation: Restriction Mapping 1. Cloned DNA can be cut with restriction enzymes and electrophoresed on agarose gels and visualized with ethidium bromide, in order to map its restriction sites (Figure 8.13). 2.
Date added: June 5, 2012 - Views: 25
Fluorescence in situ Hybridization (FISH ... method for confirming the presence of a DNA segment within an entire genome than other conventional methods like Southern blotting FISH Procedure Denature the chromosomes Denature the probe Hybridization Fluorescence staining Examine slides or ...
Date added: February 17, 2013 - Views: 188
... differences in these sequences can be used to distinguish individuals sequences are separated by gel electrophoresis Southern blotting transfers separated fragments to nylon membrane Hybridization ... Protein Synthesis Animation Biostudio Protein Synthesis Animation Polymerase Chain ...
Date added: November 29, 2013 - Views: 3
Southern blotting: Transfer DNA from ... Southern Blot Animation http://www.sanger.ac.uk/Info/Intro/gfx/fred_bw.jpg Chain termination resulting in different DNA fragment lengths Separate different DNA lengths by gel electrophoresis, ...
Date added: May 20, 2014 - Views: 1
... Retrotransposon movement Animation Quiz 5 - Transposons: ... If the probe binds to the membrane, then the probe sequence is present in the sample. Southern Blotting DNA Sequencing Determination of nucleotide sequences (Sanger method, ...
Date added: February 5, 2014 - Views: 7
... go to Animation and ... sequence Target sequence Primers New nucleo- tides 3 3 3 3 5 5 5 1 2 3 Gel Electrophoresis and Southern Blotting One indirect method of rapidly analyzing and comparing genomes is gel electrophoresis This technique uses a gel as a ...
Date added: August 20, 2014 - Views: 1
... Or DNA can be removed from the gel by Southern Blotting ... Recombinant DNA and Biotechnology,2nd ed. ASM Press, Washington Turner, P.C., et al, 1997, Instant Notes ... (it is actually quite stiff) The gel is then visualized - UV light causes the bands of DNA to fluoresce Animation ...
Date added: July 6, 2013 - Views: 7
* Southern Blotting Method * Method DNA cut with R.E. into small fragments Separated by gel electrophoresis Transferred from gel to ... * PCR animation AnimationPolymerase Chain Reaction * Applications of PCR Used by police when have small piece of ... Definition of a Gene Author: Remco Baars ...
Date added: September 18, 2013 - Views: 3
... of DNA molecules Such analysis can rapidly provide comparative information about DNA sequences Gel Electrophoresis and Southern Blotting One indirect method of rapidly analyzing and comparing genomes is gel ... Recombinant DNA molecule Sticky end Animation: Restriction Enzymes ...
Date added: May 13, 2013 - Views: 3
FISH, Southern transfer (blotting) and DNA microarray analyses involve hybridization. Hybridization. Hybridization(1) Fluorescent in situ Hybridization. FISH. Use DNA or RNA probes for hybridization. ... Animation. 1-Endo-2-Exo-Cleave . Middle of Chain.
Date added: February 5, 2012 - Views: 48
Animation file made by Justin H. Lo may help you understand how replication is related with homologous recombination: ... (Southern blotting). (A) ...
Date added: June 2, 2013 - Views: 5
See Chapter 9 animation http://bcs.whfreeman.com/lodish7e/#800911__811944__ Fig. 9.3 ... saliva, bone or any other tissue (often found at a crime scene) Can be done by southern blotting with an appropriate probe or by a PCR method using appropriate primers Can use single locus probes ...
Date added: January 21, 2013 - Views: 34
Southern blotting, DNA chips, and. FISH . Nucleic Acid Hybridization: DNA Chip. Fig 10.17. Fluorescent In Situ ... Add DNA or RNA probe attached to fluorescent dye. for S. aureus. Dichotomous Key. ANIMATION Dichotomous Keys: Practice. ANIMATION Dichotomous Keys: Sample with Flowchart. ANIMATION ...
Date added: December 26, 2012 - Views: 31
Figure 11.2 Agarose gel electrophoresis of DNA. Figure 11.4 Southern blotting. Figure 11.5 Major steps in gene cloning. ... gene libraries made by cloning random genome fragments © 2012 Pearson Education, Inc. Animation: ...
Date added: October 6, 2012 - Views: 57
AP Biology Ch. 20 Slides for Test DNA ... as two alleles for a gene The procedure is also used to prepare pure samples of individual fragments A technique called Southern blotting combines gel electrophoresis of DNA fragments with nucleic acid hybridization Specific DNA fragments can ... go to ...
Date added: March 14, 2012 - Views: 36
... Operon Induction: Biointeractive Animations: Animated Tutorials: Molecular Biology: Processing of Gene Information Animation Prokaryotes ... patterns Interactive Animation, Simplified Blotting Goes from the gel to filter paper to make and autoradiograph Southern blot ...
Date added: March 4, 2012 - Views: 41
... electrophoretically separated DNA can be screened for fragments of interest using hybridization techniques Northern and Southern blotting are two of ... you can collect your normal and cancer cells and extract the gene expression products – mRNA Animation Denaturation (94°C ...
Date added: August 5, 2013 - Views: 12
Other Applications of DNA Fingerprinting Parentage test Endangered species or Chinese herbs identification Animation 1: Southern Blotting http://www.dnalc.org/resources/BiologyAnimationLibrary.htm Animation 2: ...
Date added: March 16, 2012 - Views: 19
to a probe Southerng Blotting fig* Sumanas, Inc. animation - DNA electrophoresis & blotting * one can detect ... (recognition sequence: 5'-C^TNAG-3') Southern blotting probe: fragment of ß-globin coding ...
Date added: October 15, 2011 - Views: 39
For example, Western blotting ... bind RNA polymerase & initiate transcription http://www-class.unl.edu/biochem/gp2/m_biology/animation/gene/gene_a2 ... Signal Transduction Pathways TGF-β Gene Therapy Immunotherapy Herceptin Chemotherapy Gleevac Gene Therapy Southern Blotting ...
Date added: August 3, 2013 - Views: 16
Genetic testing for CF ... gel electrophoresis DNA fragments loaded onto a gel Current is applied DNA molecules carry a residual –ve charge See animation Activity 2.20 Fragments of DNA molecules attracted and move towards + ve electrode ... This is done by ‘Southern Blotting’. Filter ...
Date added: December 26, 2013 - Views: 8
... * * * * * Applications of Molecular Bio-techniques in Medical Oncology Molecular Biology for Medical Oncology Diagnosis Cancer screening and early detection Evaluation ... Library.htm Animation Library Thank You ... Bio-techniques Blotting Southern ...
Date added: May 23, 2013 - Views: 22
* Immunological Diagnostic Procedures Immunological diagnostic procedures are often used to ... * ELISA * ELISA Animation * ELISA Lab/Detection of HIV * Detection of HIV * DNA ... Digested DNA is separated by agarose gel electrophoresis. DNA is transferred by Southern blotting to a ...
Date added: November 18, 2013 - Views: 9