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SOUTHERN BLOTTING - Universitas Gadjah Mada

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SOUTHERN BLOTTING - Universitas Gadjah Mada

SOUTHERN BLOTTING The technique was developed by E.M. Southern in 1975. ... Would you watch the animation? A. Yes B. No SUMMARY OF PROCEDURE 1. Extract and purify DNA from cells 2. DNA is restricted with enzymes 3. Sort by electrophoresis 4. Denature DNA 5. Transfer to nitrocellulose paper 6.

http://faperta.ugm.ac.id/download/pak_tar/genetika-molekuler/presentasi/SOUTHERN%20BLOTTING.ppt

Date added: June 25, 2012 - Views: 158

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Introduction to Northern/Southern Blotting

Introduction to Southern Hybridization Michael Melzer Plant & Environmental Protection Sciences University of Hawaii at Manoa Outline History/Background Info Goals of Southern hybridization Example Other applications History/Background ‘Southern’ hybridization named after Sir Edwin Southern ...

http://abe.leeward.hawaii.edu/WebSharing/Southern_Melzer.ppt

Date added: November 6, 2011 - Views: 216

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Southern Blotting - Lakeside School

Southern Blotting INVENTED BY BRITISH BIOCHEMIST EDWIN SOUTHERN * Purpose Used to determine whether a particular nucleotide sequence is present in different sources of DNA, and also to discover the size of the restriction fragment that contains the specific sequence Example Sickle Cell Can ...

http://teachers.lakesideschool.org/us/science/parry/documents/SouthernNorthernandWesternBlotting.ppt

Date added: November 28, 2011 - Views: 234

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Genetics Techniques: RFLP & PCR - fuhsd

Southern Blotting A method to visualize specific segments of DNA– usually a particular gene. Uses radioactive probes that bind to the specific DNA segments Ex. ... DNA is completely unzipped, no Okazaki fragments PCR animation animation Genetics Techniques: ...

http://web.fuhsd.org/pamela_chow/apbio/lectures/DNA%20Replication%20&%20Genetics/Genetics%20Techniques.ppt

Date added: April 15, 2012 - Views: 298

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VNTR, STR and RFLP - Austin Community College District

Size determined by gel electrophoresis and Southern blotting to produce pattern of ... Activities Review tutorial on Western Blot http://tinyurl.com/awaut3 Review VNTR animation http://tinyurl.com/dawjau Review DNA fingerprinting http://tinyurl.com/co2yml Review DNA forensics Problem set 1 ...

http://www.austincc.edu/mlt/mdtech/BlottingMethods.ppt

Date added: September 13, 2011 - Views: 165

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الشريحة 1

Steps in Southern Blotting Southern Blotting Animation Advantages and Disadvantages of Southern Blotting Advantages The exact location of a disorder does not need to be known Disadvantages They are rather time consuming Needs high quality/quantity DNA Radioactive Some tasks previously ...

http://site.iugaza.edu.ps/mzaharna/files/2010/06/Exp.-7-Restriction-enzymes-Southern-blotting-mod.ppt

Date added: March 29, 2013 - Views: 5

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Genetic engineering and the Human Genome Project

Using living microorganisms or cell components to make products Often via genetic engineering and using ... Autoradiography pinpoints sequence Southern Blot animation The scientific applications Understanding of DNA ... is gel electrophoresis? What is Southern blotting?

http://skywalker.cochise.edu/ashraft/web/BIO205%20ppt/Lectures%202010/9%20biotechnology.ppt

Date added: May 22, 2013 - Views: 19

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… Get Document - Gavilan College -> Faculty / Staff Web Pages

Figure 20.9 Gel electrophoresis Figure 20.9 Gel electrophoresis Figure 20.11 Southern blotting of DNA fragments Figure ... allele Probe base-pairs with fragments Nitrocellulose blot 4 5 Radioactively labeled probe for -globin gene Southern blotting animation Northern blotting ...

http://hhh.gavilan.edu/rmorales/documents/20_Lecture_Presentationshortversion.ppt

Date added: September 4, 2011 - Views: 101

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DNA technology the study of sequence, expression, and ...

DNA technology the study of sequence, expression, and function of a gene DNA cloning allows researchers to Compare genes and alleles between individuals

http://mf011-jan11.wikispaces.com/file/view/MF011_fhs_lnt_003b_Jan11.ppt

Date added: May 4, 2013 - Views: 15

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PowerPoint Presentation

Scene: Animation of labeled probe fragment pairing with target ... Animation of DNA/RNA probe preparations Animation of Complementary Base-Pairing Live shot of Researcher--Segue to Blotting Southern Blot Transfer Preparation Animation of Transfer Process Crosslink of DNA to Nitrocellulose ...

http://multimedia.mcb.harvard.edu/powerpoint/No-So.ppt

Date added: November 7, 2011 - Views: 30

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Microbiology - Des Moines Area Community College, Iowa ...

... DNA Sequencing organisms' genomes DNA fingerprinting for identification Southern Blotting Southern Blotting Southern Blotting Forensic Microbiology PCR Primer for a specific organism will cause application if ... Recombinant DNA Technology Table 9.1 Figure 9.2 Figure 9.3 ANIMATION PCR: ...

http://www.library.dmacc.cc.ia.us/instructors/ashelgeson/A_PowerPoint/a_Lecture_Outline/_START-HERE_ch09_lecture.ppt

Date added: October 26, 2011 - Views: 76

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PowerPoint Presentation

Web animation of a cDNA microarray experiment Yeast genome on a chip Brief outline of steps for ... (e.g. by X-ray for radioactive probes) Sample Autoradiogragh (Gel) Types of Blots Southern ... 3’ 3’ – CTTAAG – 5’ (cut) The Southern blotting technique * Title ...

http://statwww.epfl.ch/davison/teaching/Microarrays/lec/week02.ppt

Date added: October 15, 2011 - Views: 94

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Chapter 20

... the role of a gene in an organism Several techniques are used to analyze the DNA of genes Gel Electrophoresis and Southern Blotting One indirect method of rapidly analyzing and comparing genomes is gel electrophoresis This technique uses a gel as a ... Base pairing occurs. 2 Animation: ...

http://www.course-notes.org/sites/www.course-notes.org/files/past/20_Lecture_Presentation_0.ppt

Date added: May 13, 2013 - Views: 11

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Forensic DNA Analysis

... shorter and less susceptible to degradation, first loci to be used in forensic analysis Short Tandem Repeats repeats of 2 to 7 bases ... This would take about 3 hours to perform in lab. PCR Animation DNA Fingerprinting Used to identify individuals by their ... (Southern blotting) ...

http://webpages.sou.edu/~chapman/CH300S10/Forensic%20DNA%20Analysis%20(student).ppt

Date added: February 13, 2013 - Views: 34

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Chapter 20

Chapter 20 Biotechnology ... and sickle-cell alleles of the β-globin gene * * Figure 20.11 Southern blotting of DNA fragments * Figure 20.11 Southern blotting of DNA fragments * Figure 20 ... producing fragments with “sticky ends” that bond with complementary sticky ends of other fragments ...

http://www.etsu.edu/coe/uschool/faculty/tadlockd/apbiology/chp_20_pwrpt.ppt

Date added: May 3, 2014 - Views: 5

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PowerPoint Presentation

* * Figure 20.11 Research Method: Southern Blotting of DNA Fragments * * Figure 20.12 Research Method: ... The fragments are mixed, and DNA ligase is added to bond the fragment sticky ends © 2011 Pearson Education, Inc. Animation: ...

http://faculty.gfcmsu.edu/rpeffer/BIOB%20160/Lecture%20Notes/20_Lecture_Presentation.ppt

Date added: March 16, 2013 - Views: 12

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PowerPoint Presentation

Examples of Applications: Southern blotting, DNA chips, and FISH Figs. 10.17 and 10.18 PLAY Animation: Dichotomous Keys (The Microbiology Place) Method used to establish cladogram for microorganisms? ...

http://lpc1.clpccd.cc.ca.us/lpc/zingg/Micro/lecture%20notes/M_T_Ch10_Classification_s.ppt

Date added: June 12, 2012 - Views: 65

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Biotechnology and Genetic Engineering-PBIO 450/550

PBIO 427/527: Molecular Genetics Lecture 3 - Review Southern, Northern & Western blotting DNA sequencing Polymerase chain reaction (PCR) DNA microarrays

http://www.ohio.edu/plantbio/staff/showalte/PBIO%20427%20&%20527/PBIO427%20Lecture%203%202009.ppt

Date added: May 4, 2013 - Views: 6

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PowerPoint Presentation

Examples of Applications: Southern blotting, DNA chips, and FISH Nucleic Acid Hybridization: DNA Chip Fig 10.17 Fluorescent In Situ Hybridization (FISH) Fig 10 ... Practice ANIMATION Dichotomous Keys: ...

http://lpc1.clpccd.cc.ca.us/lpc/zingg/Micro/lecture%20notes/M_T_Ch10_Classification_SS10.ppt

Date added: December 5, 2011 - Views: 74

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Chapter 20 - SCT JJ's Sciences

Several techniques are used to analyze the DNA of genes: Gel Electrophoresis, restriction fragment analysis, Southern blotting, DNA sequencing. ... Figure 20.18 Reproductive cloning of a mammal by nuclear transplantation For the Discovery Video Cloning, go to Animation and Video Files.

http://jj-sct-bio.weebly.com/uploads/2/4/6/5/2465445/20_lecture_presentation.ppt

Date added: February 9, 2012 - Views: 35

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Introduction to Biotechnology

Hybridization DNA hybridization Southern blotting (pg75) Southern Blot ... Primer extension Fig. 3.8 The Polymerase Chain Reaction Go to Animation courtesy of the following websites: References http://faculty.plattsburgh.edu/donald.slish/PCRmov.html ...

http://science.kennesaw.edu/~pachar/Btech3100/Chapter%203%20CONTINUE%20Recombinant%20DNA%20Technology.ppt

Date added: July 1, 2012 - Views: 19

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DNA Technology - Windsor Central High School

DNA Technology AP Biology ... Biology Animation Library/DNA Restriction Restriction enzymes are used to make recombinant DNA Restriction enzymes cut both strands, often in a staggered way creating single-stranded ends, ... Southern Blotting: ...

http://www.windsor-csd.org/Downloads/DNA%20Technology.ppt

Date added: April 23, 2014 - Views: 4

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Microbiology - Hillsborough Community College

... t tell you it’s classified as gammaproteobacteria References What is in Bergey’s Manual? 10-13 A Clinical Microbiology Lab Report Form Identification ... Cladogram Building a Cladogram What is tested in Western blotting and Southern ... ANIMATION Dichotomous Keys ...

http://www.hccfl.edu/media/700873/_start-here_ch10_lecture.ppt

Date added: May 3, 2013 - Views: 37

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Microbiology

... Scientific Applications Understanding DNA Sequencing organisms' genomes DNA fingerprinting for identification Southern Blotting Southern Blotting Southern Blotting Forensic Microbiology PCR Primer for a specific ... Recombinant DNA Technology Table 9.1 Figure 9.2 Figure 9.3 ANIMATION ...

http://faculty.imperial.edu/samuel.david/bio-220/item/download/56

Date added: November 27, 2013 - Views: 4

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Restriction Fragment Length Polymorphism

Restriction Fragment Length ... and gel electrophoresis DNA transfer by Southern blotting DNA hybridisation Interpreting RFLP bands Advantages and disadvantages of RFLPs ... for choosing a RFLP marker Slide 14 Summary Animation RFLP Analysis Restriction enzymes ...

http://xa.yimg.com/kq/groups/18730409/1826348150/name/Restriction

Date added: May 4, 2013 - Views: 16

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Slide 1

Polymerase Chain Reaction Animation PCR http://www.dnalc.org/ddnalc/resources/pcr.html Gel electrophoresis a method that ... http://207.207.4.198/pub/flash/4/4.html `http://www.dnalc.org/ddnalc/resources/electrophoresis.html Southern blotting Southern blotting was named after Edward M ...

http://utechdmd2015.wikispaces.com/file/view/unit4_Molecular_Biology_techniques_revised_1_.ppt

Date added: March 22, 2012 - Views: 33

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Microbiology - Des Moines Area Community College

... 9-16 Applications of rDNA 9-17 Diagram the Southern blotting ... filter DNA transferred to filter Gel Figure 9.16 Southern blotting. Forensic Microbiology PCR Primer for a specific organism will cause ... Sequence DNA Diagnose genetic disease Detect pathogens ANIMATION ...

http://www.dmacc.edu/instructors/ashelgeson/_ch_09_lecture_presentation.ppt

Date added: May 2, 2013 - Views: 29

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CHAPTER 8 Recombinant DNA Technology

Southern blotting c. Northern blotting Restriction Mapping Animation: Restriction Mapping 1. Cloned DNA can be cut with restriction enzymes and electrophoresed on agarose gels and visualized with ethidium bromide, in order to map its restriction sites (Figure 8.13). 2.

http://grassland.agron.ntu.edu.tw/genelab/Class_slide/Peter_Russell/2Ed/Ch8.ppt

Date added: June 5, 2012 - Views: 25

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No Slide Title

Fluorescence in situ Hybridization (FISH ... method for confirming the presence of a DNA segment within an entire genome than other conventional methods like Southern blotting FISH Procedure Denature the chromosomes Denature the probe Hybridization Fluorescence staining Examine slides or ...

http://www3.nd.edu/~ljordan/butterfly/genetics/fish.ppt

Date added: February 17, 2013 - Views: 188

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Chapter 12 DNA and RNA

... differences in these sequences can be used to distinguish individuals sequences are separated by gel electrophoresis Southern blotting transfers separated fragments to nylon membrane Hybridization ... Protein Synthesis Animation Biostudio Protein Synthesis Animation Polymerase Chain ...

http://mr-ippolito-science-class.org/files/F_Chapter9DNATheIndispensibleForensicTool200809.pps

Date added: November 29, 2013 - Views: 3

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PowerPoint Presentation

Southern blotting: Transfer DNA from ... Southern Blot Animation http://www.sanger.ac.uk/Info/Intro/gfx/fred_bw.jpg Chain termination resulting in different DNA fragment lengths Separate different DNA lengths by gel electrophoresis, ...

http://fmmh.ycdsb.ca/teachers/fmmh_dsilva/pages/SBI%203U7/FOV1-00072661/4.4%20part%203.ppt

Date added: May 20, 2014 - Views: 1

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PowerPoint Presentation

... Retrotransposon movement Animation Quiz 5 - Transposons: ... If the probe binds to the membrane, then the probe sequence is present in the sample. Southern Blotting DNA Sequencing Determination of nucleotide sequences (Sanger method, ...

http://faculty.muhs.edu/klestinski/18-19-20.ppt

Date added: February 5, 2014 - Views: 7

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Chapter 20

... go to Animation and ... sequence Target sequence Primers New nucleo- tides 3 3 3 3 5 5 5 1 2 3 Gel Electrophoresis and Southern Blotting One indirect method of rapidly analyzing and comparing genomes is gel electrophoresis This technique uses a gel as a ...

http://fhsapbio.weebly.com/uploads/1/3/5/3/13535409/ap_bio_ch20_lecture_presentation.ppt

Date added: August 20, 2014 - Views: 1

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Gel Electrophoresis of DNA - Universitas Gadjah Mada

... Or DNA can be removed from the gel by Southern Blotting ... Recombinant DNA and Biotechnology,2nd ed. ASM Press, Washington Turner, P.C., et al, 1997, Instant Notes ... (it is actually quite stiff) The gel is then visualized - UV light causes the bands of DNA to fluoresce Animation ...

http://faperta.ugm.ac.id/newbie/download/pak_tar/genetika-molekuler/presentasi/elpho.ppt

Date added: July 6, 2013 - Views: 7

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Definition of a Gene

* Southern Blotting Method * Method DNA cut with R.E. into small fragments Separated by gel electrophoresis Transferred from gel to ... * PCR animation AnimationPolymerase Chain Reaction * Applications of PCR Used by police when have small piece of ... Definition of a Gene Author: Remco Baars ...

http://students.kaikourahigh.school.nz/biology/13resources/biotechnology.ppt

Date added: September 18, 2013 - Views: 3

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video slide

... of DNA molecules Such analysis can rapidly provide comparative information about DNA sequences Gel Electrophoresis and Southern Blotting One indirect method of rapidly analyzing and comparing genomes is gel ... Recombinant DNA molecule Sticky end Animation: Restriction Enzymes ...

http://www.morgancc.edu/faculty/Smith,L/Biology/Power%20Points/Biology%20Ch%2018/20_Lectures_PPT/20_Lectures_PPT%20.ppt

Date added: May 13, 2013 - Views: 3

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Biochemical Properties of DNA and The Technology involve them

FISH, Southern transfer (blotting) and DNA microarray analyses involve hybridization. Hybridization. Hybridization(1) Fluorescent in situ Hybridization. FISH. Use DNA or RNA probes for hybridization. ... Animation. 1-Endo-2-Exo-Cleave . Middle of Chain.

http://faculty.ksu.edu.sa/2475/Lectures1/4-Biochemical%20Properties%20of%20DNA%20and%20The%20Technology%20involve%20them.pptx

Date added: February 5, 2012 - Views: 48

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Rad51-deficient vertebrate cells accumulate chromosomal ...

Animation file made by Justin H. Lo may help you understand how replication is related with homologous recombination: ... (Southern blotting). (A) ...

http://openwetware.org/images/d/d4/Manuscript_Discussion.ppt

Date added: June 2, 2013 - Views: 5

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Biotechnology and Genetic Engineering-PBIO 450/550

See Chapter 9 animation http://bcs.whfreeman.com/lodish7e/#800911__811944__ Fig. 9.3 ... saliva, bone or any other tissue (often found at a crime scene) Can be done by southern blotting with an appropriate probe or by a PCR method using appropriate primers Can use single locus probes ...

http://www.ohio.edu/plantbio/staff/showalte/PBIO%20450%20&%20550/PBIO4500%20Molecular%20Diagnostics.ppt

Date added: January 21, 2013 - Views: 34

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PowerPoint Presentation

Southern blotting, DNA chips, and. FISH . Nucleic Acid Hybridization: DNA Chip. Fig 10.17. Fluorescent In Situ ... Add DNA or RNA probe attached to fluorescent dye. for S. aureus. Dichotomous Key. ANIMATION Dichotomous Keys: Practice. ANIMATION Dichotomous Keys: Sample with Flowchart. ANIMATION ...

http://ashomarconfidential.files.wordpress.com/2011/11/introduction-to-systematic-bacteriology.pptx

Date added: December 26, 2012 - Views: 31

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PowerPoint Presentation

Figure 11.2 Agarose gel electrophoresis of DNA. Figure 11.4 Southern blotting. Figure 11.5 Major steps in gene cloning. ... gene libraries made by cloning random genome fragments © 2012 Pearson Education, Inc. Animation: ...

http://bio.illinoisstate.edu/wanicho/bsc260/ppt/genetic%20engineering.ppt

Date added: October 6, 2012 - Views: 57

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AP Biology Ch. 20 - PBworks

AP Biology Ch. 20 Slides for Test DNA ... as two alleles for a gene The procedure is also used to prepare pure samples of individual fragments A technique called Southern blotting combines gel electrophoresis of DNA fragments with nucleic acid hybridization Specific DNA fragments can ... go to ...

http://apbiologyclass.pbworks.com/f/AP+Bio+Ch.+20.ppt

Date added: March 14, 2012 - Views: 36

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DNA Technology - Dr. Annette M. Parrott

... Operon Induction: Biointeractive Animations: Animated Tutorials: Molecular Biology: Processing of Gene Information Animation Prokaryotes ... patterns Interactive Animation, Simplified Blotting Goes from the gel to filter paper to make and autoradiograph Southern blot ...

http://www.africangreyparrott.com/DNATechnology.ppt

Date added: March 4, 2012 - Views: 41

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How Genes and Genomes Evolve - West Virginia University

... electrophoretically separated DNA can be screened for fragments of interest using hybridization techniques Northern and Southern blotting are two of ... you can collect your normal and cancer cells and extract the gene expression products – mRNA Animation Denaturation (94°C ...

http://www.as.wvu.edu/~dray/219files/BIO219_Chapter_10_11-9-05_DAR.ppt

Date added: August 5, 2013 - Views: 12

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PowerPoint Presentation

Other Applications of DNA Fingerprinting Parentage test Endangered species or Chinese herbs identification Animation 1: Southern Blotting http://www.dnalc.org/resources/BiologyAnimationLibrary.htm Animation 2: ...

http://www.chuenyuen.edu.hk/~cyc-bio/docs/AL/Electrophoresis%20by%20CUHK.ppt

Date added: March 16, 2012 - Views: 19

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MICRO. 555 (555 Microbial Molecular Genetics) Dr.Afaf Ibrahim ...

to a probe Southerng Blotting fig* Sumanas, Inc. animation - DNA electrophoresis & blotting * one can detect ... (recognition sequence: 5'-C^TNAG-3') Southern blotting probe: fragment of ß-globin coding ...

http://faculty.ksu.edu.sa/dr.afafshehata/Lectures/Mic.555/PowerPoint%20%20Mic%20555%20(2).ppt

Date added: October 15, 2011 - Views: 39

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Molecular & Genomic Surgery - The Medical University of South ...

For example, Western blotting ... bind RNA polymerase & initiate transcription http://www-class.unl.edu/biochem/gp2/m_biology/animation/gene/gene_a2 ... Signal Transduction Pathways TGF-β Gene Therapy Immunotherapy Herceptin Chemotherapy Gleevac Gene Therapy Southern Blotting ...

http://academicdepartments.musc.edu/surgery/education/basic_science/2009-10/molecular_genomic_surgery.pps

Date added: August 3, 2013 - Views: 16

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Genetic testing for CF - intranet

Genetic testing for CF ... gel electrophoresis DNA fragments loaded onto a gel Current is applied DNA molecules carry a residual –ve charge See animation Activity 2.20 Fragments of DNA molecules attracted and move towards + ve electrode ... This is done by ‘Southern Blotting’. Filter ...

http://intranet.wellingtoncollege.org.uk/resource.aspx?id=2724

Date added: December 26, 2013 - Views: 8

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Genetics - جامعة الملك عبد العزيز | جدة ...

... * * * * * Applications of Molecular Bio-techniques in Medical Oncology Molecular Biology for Medical Oncology Diagnosis Cancer screening and early detection Evaluation ... Library.htm Animation Library Thank You ... Bio-techniques Blotting Southern ...

http://www.kau.edu.sa/Files/140/Files/30721_Molecular%20medicine.ppt

Date added: May 23, 2013 - Views: 22

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DIAGNOSTICS AND BIOMARKERS - muhammad1988adeel

* Immunological Diagnostic Procedures Immunological diagnostic procedures are often used to ... * ELISA * ELISA Animation * ELISA Lab/Detection of HIV * Detection of HIV * DNA ... Digested DNA is separated by agarose gel electrophoresis. DNA is transferred by Southern blotting to a ...

http://muhammad1988adeel.files.wordpress.com/2011/10/biot-412-11-dx-and-biomarkers.ppt

Date added: November 18, 2013 - Views: 9