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Combined Cosmids (ppt) - University of Illinois at Chicago - UIC

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Combined Cosmids (ppt) - University of Illinois at Chicago - UIC

Alcaligenes faecalis combined cosmids map with 70 ORFs Amino acid permease L-Kynurenine hydrolase Transcriptional regulators orf77 gntR orf416 orf153

http://www.uic.edu/depts/mcmi/faculty/silver/Combined%20Cosmids%20Map.ppt

Date added: October 24, 2011 - Views: 50

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PowerPoint Presentation

cosmids Cosmids are plasmid vectors that contain one or two “cos sites”. (The cos site and the length between 2 cos sites is the only requirement for DNA to be

http://genemol.org/molecular-bio/7-Artificial-chromosomes/YAC-BAC-PAC.ppt

Date added: October 7, 2011 - Views: 202

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Vectors - College of Computer, Mathematical, and Natural Sciences

Cosmids are extracted from bacteria and mixed with restriction endonucleases. Cleaved cosmids are mixed with foreign DNA that has been cleaved with the same endonuclease.

http://www.life.umd.edu/grad/MLfsc/res/RecombomamtDNA&Medicine.ppt

Date added: July 18, 2013 - Views: 32

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PowerPoint Presentation

Cosmids, however, always form colonies and not plaques. Also the clone density is much lower with around 105 - 106 CFU per µg of ligated DNA.

http://forest.mtu.edu/classes/fw3300/BL3300%202011/2011%20%20Chapt%2003%20091904%20cpj.ppt

Date added: May 21, 2012 - Views: 42

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Introduction to Vectors - Rutgers University

Title: Introduction to Vectors Last modified by: Bryce Nickels Document presentation format: On-screen Show (4:3) Company: Rutgers University Other titles

http://beadle.rutgers.edu/MBB/315/Lec/315-Ch2.ppt

Date added: November 12, 2011 - Views: 96

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PowerPoint Presentation

Vectors for large DNA inserts: Examples: Cosmids PACs BACs Plasmid Vectors Origin of Replication b-lactamase: breaks down ampicillin ...

http://faculty.smu.edu/borr/ClassNotes/10-14-05.ppt

Date added: May 13, 2013 - Views: 18

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justc/courses/RecombDNA/Week6. ppt

Cosmids BACs PACs YACs Cosmids replicate as high copy number plasmids--tend to be unstable, deleting insert DNA (to reduce drag on cells) ...

http://web.pdx.edu/~justc/courses/RecombDNA/Week6.ppt

Date added: October 7, 2011 - Views: 64

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PowerPoint Presentation

2-6 2-7 Life cycle of a bacteriophage 2-8 Constructing a genomic library in phage 2-9 Constructing a genomic library in cosmids 2-10 Constructing a genomic library in YACs * Look at vector first, Where are all the the sites that you know about? * * *

http://wssp.rutgers.edu/StudentScholars/WSSP06/WSSP-06_files/Figures/WSSP06-Ch2-LIbraries.ppt

Date added: May 5, 2013 - Views: 24

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PowerPoint Presentation

Other vectors include bacteriophage, cosmids, etc. pUC18 Plasmid Origin of replication DNA fragment up to 5 kbp can insert IV. Cloning with a Plasmid Vector Recombinant DNA Transformation Selection for cells carrying recombinant plasmids by plating cells on media with antibiotic.

http://people.cst.cmich.edu/schis1j/bio326-02/lectures/11-8.ppt

Date added: January 13, 2013 - Views: 12

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PowerPoint Presentation

... & Cosmids (plasmid + l cos site & can be pckged into phage capsid) Transgenic Plant: Vector = Agrobacterium tumefaciens plasmid called Ti (causes crown gall tumors). Plasmid codes for virulence gene and regulates pant growth ...

http://www.oucom.ohiou.edu/dbms-james/Winter_2003/Recomb%20DNA%20Tech.ppt

Date added: August 2, 2013 - Views: 12

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PowerPoint Presentation

Fosmids offer higher stability than comparable high copy number cosmids. Contain other features similar to plasmids/cosmids such as origin sequence and polylinker. Recombinant DNA Libraries (2 major types): Genomic/chromosomal library, ...

http://mercury2.iab.uaf.edu/kevin_mccracken/genetics/lectures/chapter_08.ppt

Date added: August 31, 2013 - Views: 3

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PowerPoint Presentation - DNA Libraries - Biology Department ...

Title: PowerPoint Presentation - DNA Libraries Author: Jeffery Young Last modified by: Jeff Young Created Date: 10/23/2009 5:04:36 PM Document presentation format

http://www.biol.wwu.edu/young/321/lectures/lg8.ppt

Date added: October 6, 2011 - Views: 128

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PowerPoint Presentation

... screening labelling PowerPoint Presentation PowerPoint Presentation phage and cosmid screening phages cosmids PACs BACs PowerPoint Presentation YACs PowerPoint Presentation PowerPoint Presentation Shotgun sequencing Chromosome Walking Chromosome jumping. Southern (DNA) and ...

http://genemol.org/molecular-bio/technics.ppt

Date added: October 12, 2011 - Views: 17

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Lecture 16: Genomics - University of Alabama at Birmingham

Genomics The Human Genome Project Mapping and Sequencing the Genomes of Model Organisms Data Collection and Distribution Ethical, Legal, ... 200 Kb Intermediate Cosmids Lambda clones Small Plasmids; ...

http://www.genome.uab.edu/MIC753/files/16_Genome.ppt

Date added: November 6, 2011 - Views: 83

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Plasmids - e-campus21

Plasmids (Objectives of this Lecture) Understand the basic structures of plasmids Know the types of plasmids and their forms Undertand the methods used to transform E coli

http://www.e-campus21.com/courseware/teachaids/bmg/mg1/mg1/plasmids.ppt

Date added: May 3, 2013 - Views: 47

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PowerPoint Presentation

Bacteriophage-based Cosmids: Linear DNA molecules used for genomic and cDNA cloning. Bacterial host. Insert size range < 20kb. Bacterial Artificial Chromosomes (BACs): Circular DNA molecules used for cloning very long segments of genomic DNA. Bacterial ...

http://users.umassmed.edu/maria.zapp/Block2Lecture1%2011_01_04.ppt

Date added: October 7, 2011 - Views: 103

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PowerPoint Presentation

... Life cycle of a bacteriophage 2-8 Constructing a genomic library in phage 2-9 Constructing a genomic library in cosmids 2-10 Constructing a genomic library in YACs * Look at vector first, Where are all the the sites that you know about? *

http://www.pascack.k12.nj.us/cms/lib5/NJ01000238/Centricity/Domain/142/WSSP06-Ch2-LIbraries.ppt

Date added: August 8, 2014 - Views: 1

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PowerPoint Presentation

Mobilizing DNA: vectors for propagation in E. coli Plasmids Bacteriophage M13 Lambda Cosmids and BACs * 5´ ... G^A A T T C ... 3´ 3´ ... C T T A A^G ... 5´ 5´ ... G^ 3’ 5’ A A T T C ... 3´ 3´ ... C T T A A 5 ...

http://web.pdx.edu/~justc/courses/RecombDNA/Week4.ppt

Date added: October 3, 2011 - Views: 88

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Chapter 12

Plasmid Placement in Cell PowerPoint Presentation Phage vectors Phage Insertion Cosmids YACs YACs YACs Essential elements for YACs PowerPoint Presentation Particle Gun We can insert the gene into cells – Now what? Basic Steps ...

http://www3.nd.edu/~ljordan/butterfly/genetics/biotech.ppt

Date added: May 3, 2013 - Views: 20

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PowerPoint Presentation

... from GeneScan and Ensembl are occasionally contradictory chicken also contained small mini-chromosomes that resemble cosmids which were lumped into the category of ChrUn, ...

http://instructional1.calstatela.edu/jmomand2/2004/presentations/ppt/ng.ppt

Date added: May 6, 2013 - Views: 7

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Chapter 3

... Types of vectors Bacterial plasmid bacteriophage cosmids bacterial artificial chromosome yeast artificial chromosome 6-12 25 35 300 200-1000 Maximum insert size (kilobases or kb [1000bp]) Practical Features of DNA Cloning Vectors (Plasmids) origin of replication ...

http://www.montana.edu/wwwmb/coursehome/BIOM107CS/Lectures/Chapter%203.ppt

Date added: October 10, 2012 - Views: 42

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Biotechnology and Genetic Engineering-PBIO 450/550

MCB 7200: Molecular Biology Gene libraries cDNA libraries Library screening Eukaryotic gene organization enhancers silencers Genomic library construction Screening a genomic library using DNA hybridization to a (radio-)labeled DNA probe Note: a cDNA is commonly (radio-)labeled and used as a DNA ...

http://www.ohio.edu/plantbio/staff/showalte/MCB%20720/MCB7200%20Lecture%203.ppt

Date added: February 13, 2013 - Views: 28

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DNA cloning intro.ppt - Biology

Clone large inserts of DNA: size ~ 45 kb Features: Cosmids are Plasmids with one or two Lambda Cos sites. Presence of the Cos site permits in vitro packaging of cosmid DNA into Lambda particles COSMID VECTOR Thus, ...

http://biology.hunter.cuny.edu/molecularbio/Class%20Materials%20Fall%202012%20710.LC/ANiMATED%20PPT%20to%20help%EF%80%A8/DNA%20cloning%20intro.ppt

Date added: January 23, 2013 - Views: 29

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PowerPoint Presentation

... allow reproduction of gene plasmids phages cosmids part viral, part plasmid BACs - bacterial artificial chromosomes YACs - yeast artificial chromosomes Cloning Cloning Use SAME restriction endonuclease to cut out target gene and open vector Must use an enzyme that produces sticky ends!

http://people.uncw.edu/doddd/Biology%20335/PowerPoints/DNAtech.ppt

Date added: March 10, 2012 - Views: 16

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PowerPoint Presentation

The physical map of the C. elegans genome was put together after ordering then sequencing genomic YACs and cosmids. * The genetic and physical maps can be aligned with each other. The spacing can vary between genetic markers and their physical genes; however, ...

http://beadle.rutgers.edu/MMG/502files/502-Ce/502-Ce-L2.ppt

Date added: June 27, 2012 - Views: 15

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Part II: Recombinant DNA Technology.ppt - Cabrini College

Recombinant DNA Technology Sherry Fuller-Espie, Ph.D., DIC Associate Professor ... rDNA molecules replicate as plasmids Very large inserts can be accommodated by cosmids ... Recombinant DNA Technology.ppt Author: ITR Last modified by: Sherry Fuller-Espie Created Date:

http://www.cabrini.edu/sepchedna/2003%20Workshop/Power%20Point%20Presentations%202003/Part%20II%20Recombinant%20DNA%20Technology_NO_FIGS.ppt

Date added: October 7, 2011 - Views: 241

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Foundations in Microbiology - South Georgia College

Lecture PowerPoint to accompany Foundations in Microbiology Sixth Edition Talaro Chapter 10 Genetic Engineering: A Revolution in Molecular Biology

http://faculty.sgc.edu/sundram/2215/Chapter10_lecture.ppt

Date added: December 13, 2011 - Views: 70

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PowerPoint Presentation

... DNA splicing * * * * * * * Table 3.1 * * * Table 3.1 * * Figure 3.3b * * * Figure 3.3b * * Figure 3.5 * * * Figure 3.5 Cosmids Contain cos ends of lambda DNA, a plasmid origin of replication, and genes for antibiotic resistance, ...

http://ap-biology-lab.wikispaces.com/file/view/chapter+3.ppt

Date added: June 30, 2014 - Views: 4

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Lambda Vectors and their replication - Department Of ...

Lambda vectors and their replication Sonita Gafary Biochem 72020 Lambda was first discovered at the Pasteur Institute by Andre Lwoff when he observed strains of E. Coli.

http://biology.hunter.cuny.edu/molecularbio2/Seminar%20Presentations/February%2027/Lambda%20Vectors%20and%20their%20replication.ppt

Date added: September 30, 2013 - Views: 27

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PowerPoint Presentation

1 Plasmid vectors 2 Bacteriophage vectors 3 Cosmids 4 BACs & YACs Plasmid vectors Advantages: Small, easy to handle Straightforward selection strategies Useful for cloning small DNA fragments (< 10kbp) Disadvantages: Less useful for cloning large DNA fragments ...

http://faculty.ksu.edu.sa/73614/amustafa/355/chapter3.ppt

Date added: December 10, 2013 - Views: 7

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Plasmids and Vectors - Molecular Biology for Secondary ...

Plasmids and Vectors Instructor Supplement to pGlo Bacterial Transformation A more detailed look at plasmids Cloning into a Plasmid Asilomar Conference Screening bacteria by replica plating Cosmids Other Vectors BACs (Bacterial artificial chromosomes) Large low copy number plasmids (have ori and ...

http://unlmbsc.files.wordpress.com/2007/08/plasmids-and-vectors.ppt

Date added: October 16, 2011 - Views: 187

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Part II: Recombinant DNA Technology.ppt - Rajesh Banu

Constructing Genomic and cDNA Libraries A. Definition A cloned set of rDNA fragments representing either the entire ... rDNA molecules replicate as plasmids Very large inserts can be accommodated by cosmids ... Recombinant DNA Technology.ppt Author: ITR Last modified by: Konkuk university ...

http://rajeshbanu.com/ebt/molecular%20breeding_RDT_2.ppt

Date added: May 2, 2013 - Views: 21

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Genome Sequencing - Kennesaw State University College of ...

Genome Sequencing Genome Sequencing Bacteriophage fX174, the first genome to be sequenced, is a viral genome with only 5,368 base pairs (bp).

http://science.kennesaw.edu/~jsalern3/Site/bioinfo/Genome%20Sequencing.ppt

Date added: September 12, 2011 - Views: 43

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Cloning into Plasmids - Buffalo State College

Cloning into Plasmids Restriction Fragment Cloning & PCR Cloning by the Topo TA™ Method Cloning Vectors The molecular analysis of DNA has been made possible by the cloning of DNA.

http://faculty.buffalostate.edu/eastondp/dpeastonbiolog/Bio%20450/Cloning%20into%20Plasmids%20-Riboprobes.ppt

Date added: December 2, 2011 - Views: 83

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Molecular Cloning - University of Hawaii

Plasmids – used in cloning small segments of DNA (10-15 kb) Cosmids – plasmids containing DNA sequences (cos) from bacteriophage λ used to clone larger fragments of up to 45 Kb Bacteriophage λ ...

http://abe.leeward.hawaii.edu/Summer07/Introduction%20to%20Molecular%20Cloning.ppt

Date added: November 5, 2011 - Views: 75

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Fragile X Syndrome - University of Wisconsin–Eau Claire

Fragile X Syndrome (Martin ... Created a cosmid library from the YAC clone Cosmid subclones used to screen a cDNA library of human fetal brain RNA The cosmids hybridized to a portion of a gene designated as FMR1 Pedigree of a family with the Fragile X mutation segregating FMR1 Expression ...

http://www.uwec.edu/anderju/biol409/presentations/Fragile%20X%20Syndrome.ppt

Date added: June 20, 2012 - Views: 43

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Gene cloning - جامعة الملك عبد العزيز ...

Cosmids are extracted from bacteria and mixed with restriction endonucleases. Cleaved cosmids are mixed with foreign DNA that has been cleaved with the same endonuclease.

http://www.kau.edu.sa/Files/0002512/Subjects/Gene%20cloning3.ppt

Date added: March 19, 2014 - Views: 3

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Recombinant DNA Technology - NIU Department of Biological ...

Recombinant DNA Technology Needles in Haystacks How to find one gene in large genome? A gene might be 1/1,000,000 of the genome. Three basic approaches: 1.

http://www.bios.niu.edu/johns/humgen/Recombinant_DNA_Technology.ppt

Date added: October 7, 2011 - Views: 244

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Biotechnology and Genetic Engineering-PBIO 450/550

... to make and screen cDNA libraries Several different cloning vectors including plasmids, bacteriophage lambda (l), and cosmids ...

http://www.ohio.edu/plantbio/staff/showalte/PBIO%20450%20&%20550/PBIO450Lecture308.ppt

Date added: October 6, 2011 - Views: 21

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Chem 360 Lecture slides - Weston

Cosmids - artificially ... Radiolabeled vs. fluorescent tag Isolation of Gene of Interest Isolation of Gene of Interest Use of Antibodies Ab ppt protein Protein being synthesized on mRNA Generate ... Harpers Review of Biochemistry Manipulation of DNA Sequence Restriction enzymes ...

http://www.weston.org/schools/ms/biologyweb/heredity/handouts/recombinant_dna_tech_ppt.ppt

Date added: May 2, 2013 - Views: 29

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Incorrect Baseline settings can have an adverse affect on ...

... Default set-up and AutoSeq1 Cosmids, Bacs, Genomic: BAC DSRG Profile 96° 5min 96 ° 30 sec 50 ° 20sec 60 ° 4 min C.S. Rxn conditions DNA- 1ug 10 pmol Primer straight BDv3.1 BAC’s: Modified Set-up, BAC profile BAC Sequencing: LOR ...

http://www.uvm.edu/medicine/vtcancercenter/documents/DNASem5.11.07.ppt

Date added: July 26, 2013 - Views: 18

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Finding Disease Genes - NIU Department of Biological Sciences

Need to clone the region between these genes walking by cosmids over 500 kbp problem with uncloneable repeated sequences: needed to jump over them. Cut genomic DNA into large fragments, circularize it and clone the junction fragments.

http://www.bios.niu.edu/johns/humgen/Finding_Disease_Genes.ppt

Date added: June 28, 2012 - Views: 24

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Gene Cloning

Lecture # 04 Cloning Vectors Recombinant DNA Technology Common General Cloning Strategy Target DNA from donor organism extracted, cut with compatible restriction endonuclease and ligated into a cloning vector.

http://xa.yimg.com/kq/groups/24240295/1734630456/name/lecture+04.ppt

Date added: February 18, 2014 - Views: 6

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Section D - Prokaryotic and Eukaryotic Chromosome Structure

H3 Cosmids, YACs and BACs — Cloning large DNA fragments Analysis of eukaryotic genes and the genome organization of eukaryotes requires vectors with a larger capacity for cloned DNA than plasmids or phage Human genome (3 x 109 bp ...

http://fzswx.gxu.edu.cn/course/Section%20H%20-%20Cloning%20vectors.ppt

Date added: February 13, 2012 - Views: 10

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Recombinant DNA Technology for the non-science major.

Cosmids (artificial constructions) A prokaryotic vector should: 1. Be capable of autonomous replication independent of the main bacterial chromosome 2. Be easy to isolate, i.e. small. 3. Be non-toxic to host cells. 4. Have space ...

http://astarte.csustan.edu/~tom/bioinfo/ppt/Day3.ppt

Date added: May 8, 2014 - Views: 4

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Chapter 3

... Plating cells on agar surface to promote colony formation Cloning Types of vectors Bacterial plasmid bacteriophage cosmids bacterial artificial chromosome yeast artificial chromosome Practical Features of DNA Cloning Vectors (Plasmids) origin of replication ...

http://www.montana.edu/wwwmb/coursehome/mb105/Lectures/Chapter%203.ppt

Date added: September 27, 2011 - Views: 85

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Présentation PowerPoint - Institut Pasteur

... A. Rajandream Central Veterinary Lab. R.G. Hewinson S.V. Gordon NIH NIAID * Sequence obtained from combined approach cosmids, BA Cs and WGS 4000 genes; 60% with functions; islands with 80%GC 1 Polyketides involved in 3 diff pathologies Produced by PKS, ...

http://www.pasteur.fr/~tekaia/BCGA/TALKS/Cole_Origin_Tuberculosis.ppt

Date added: November 14, 2011 - Views: 25

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Inquiry into Life Twelfth Edition - McGraw-Hill Education

... labeled nucleic acid with sequence matching the gene of interest Cosmids Cosmids are designed for cloning large DNA fragments Behave as plasmid and phage Contain cos sites, ...

http://highered.mheducation.com/sites/dl/free/0072995246/444682/chapt04_lecture.ppt

Date added: July 14, 2014 - Views: 1

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PowerPoint Presentation

AD000092 /FEATURE=cds#6 /DEFINITION=CH19HHR23 Homo sapiens DNA from chromosome 19p13.2 cosmids R31240, R30272 and R28549 containing the EKLF, GCDH, CRTC, and RAD23A genes, genomic sequence. 1753_s_at.

http://combio.cs.brandeis.edu/hong/CS230a/CourseInfo/DetectDEG.ppt

Date added: June 22, 2012 - Views: 35

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Computational Biology Lecture #1: Introduction

These short “sequence reads” are to be combined to create sequenced DNA up to 50 Kb (Cosmids) 200 Kb (BACs) 3,000 Mb (Whole Genome) As the size of the “Sequenced DNA” scales up to the whole genome, several other issues are faced: Segmental duplications, homologies, etc. Repeats, ...

http://www.cs.nyu.edu/cs/faculty/mishra/COURSES/01.COBIO/05.cb1.ppt

Date added: August 6, 2013 - Views: 3